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KMID : 0615720020190040190
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Jouranl of Korean Acupuncture and Moxibustion society
2002 Volume.19 No. 4 p.190 ~ p.199
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Effect of Scutellaria Baicalensis Georgi Extraction(SbGE) on H_2O_2-induced Inhibition of Phosphate Transport in Renal Epithelial Cells
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Abstract
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Objective : This study was performed to determine if Scutellaria balicalensis Georgi extract (SbGE) prevents oxidant-induced membrane transport dysfunction in renal tubular cells.
Methods : Membrane transport function was estimated by measuring Na+-dependent inorganic phosphate transport in opossum kidney (OK) cells. H_2O_2 inhibited phosphate transport in a dose-dependent manner.
Results : The inhibitory effect of H_2O_2 was significantly prevented SbGE over concentration range of 0.005-0.05%. H_2O_2 caused ATP depletion, which was prevented by SbGE. H_2O_2 induced the loss of mitochondrial function as evidenced by decreased MTT reduction and its effect was prevented by SbGE. The H_2O_2-induced inhibition of phosphate transport was not affected by a potent antioxidant DPPD, but the inhibition was prevented by an iron chelator deferoxamine, suggesting that H_2O_2 inhibits Na+-dependent phosphate transport via an iron-dependent nonperoxidative mechanism in renal tubular cells.
Conclusion : These data suggest that SbGE may exert the protective effect against oxidant-induced membrane transport dysfunction by a mechanism similar to iron chelators in renal epithelial cells. However, furher studies should be carried out to find the active ingredient(s) of SbGE that exerts the protective effect.
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